A psychiatrist tells two stories: one of a trans woman, the other of a pseudohermaphrodite.
Bela Lugosi
as Scientist
Edward D. Wood Jr.
as Glen / Glenda (as Daniel Davis)
Lyle Talbot
as Inspector Warren
Timothy Farrell
as Dr. Alton / Narrator
Dolores Fuller
as Barbara
'Tommy' Haynes
as Alan / Anne
Charlie Crafts
as Johnny (as Charles Crafts)
Conrad Brooks
as Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)
Henry Bederski
as Man with Hat and Receding Hairline (uncredited)
Carol Daugherty
as Woman in Nightmare (uncredited)
'Captain' DeZita
as The Devil / Glen's Father (uncredited)
Helen Miles
as (uncredited)